Strategies to Combat Hypoxia in Encapsulated Islet Transplantation
نویسندگان
چکیده
Abbreviations: T1D: Type 1 Diabetes; PCO: Pericapsular Overgrowth; OCR: Oxygen Consumption Rate; HBO: Hyperbaric Oxygen Therapy; EX-4: Exendin-4; FGF-1: Fibroblast Growth Factor – 1; aFGF: acidic Fibroblast Growth Factor; VEGF: Vascular Endothelial Growth Factor;VEGFR2: Vascular Endothelial Growth Factor Receptor 2; AMPK: Adenosine Monophosphate Activated Protein Kinase ; NFκB: Nuclear Factor κ-light chain enhancer of activated B-Cells; PDMS: Polydimethylsiloxane; n PTFE: Polytetrafluoroethylene; HIF1α: Hypoxia-InducibleFactor-1α; HIMP1: Hypoglycemia/hypoxia Inducible Mitochondrial Protein; BNIP-3: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; Bcl-2: B-cell lymphoma 2; PDX-1: Pancreatic and duodenal homeobox 1;HO-1: Heme Oxygenase-1; HSP32: Heat Shock Protein-32; APA: Alginate-Poly-L-Lysine-Alginate; PLO: Poly-L-Ornithine; PFC: Perfluorocarbon; BAP: Bioartificial Pancreas; NPI: Neonatal Porcine Islets; LVG: Low Viscosity High Guluronate; TUNEL: Terminal deoxynucleotidyl transferase dUTP nick end labeling; UPMVG: UltraPure Medium Viscosity High Guluronate; ATP: Adenosine Triphosphate; ADP: Adenosine Diphosphate; GHRH:Growth Hormone Releasing Hormone; CYGB: Cytoglobin; COX-2: Cyclooxygenase – 2; Hb-C: Crosslinked Hemoglobin; IEQ: Islet Equivalents; NCX1: Na+-Ca++ Exchanger 1; MT 1⁄2: Metallothionein 1⁄2; MT-II: Metallothionein – II; MCP-1: Macrophage Chemoattractant Protein -1; XIAP: X-linked Inhibitor of Apoptosis Protein; MIN6: Mouse Insulinoma 6; GSIR: Glucose Stimulated Insulin Response; CaO2: Calcium Peroxide; SrCl2: Strontium Chloride; MSC: Mesenchymal Stem Cell
منابع مشابه
Alginate Microencapsulation of Human Islets Does Not Increase Susceptibility to Acute Hypoxia
Islet transplantation in diabetes is hampered by the need of life-long immunosuppression. Encapsulation provides partial immunoprotection but could possibly limit oxygen supply, a factor that may enhance hypoxia-induced beta cell death in the early posttransplantation period. Here we tested susceptibility of alginate microencapsulated human islets to experimental hypoxia (0.1-0.3% O2 for 8 h, f...
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